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Gene Gun Delivery Systems for Cancer Vaccine Approaches

  • Kandan Aravindaram
  • Ning Sun YangEmail author
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 542)

Summary

Gene-based immunization with transgenic DNA vectors expressing tumor-associated antigens (TAA), cytokines, or chemokines, alone or in combination, provides an attractive approach to increase the cytotoxic T cell immunity against various cancer diseases. With this consideration, particle-mediated or gene gun technology has been developed as a nonviral method for gene transfer into various mammalian tissues. It has been shown to induce both humoral and cell-mediated immune responses in both small and large experimental animals. A broad range of somatic cell types, including primary cultures and established cell lines, has been successfully transfected ex vivo or in vitro by gene gun technology, either as suspension or adherent cultures. Here, we show that protocols and techniques for use in gene gun-mediated transgene delivery system for skin vaccination against melanoma using tumor-associated antigen (TAA) human gp100 and reporter gene assays as experimental systems.

Keywords

DNA vaccine gene gun gp100 melanoma metastasis mouse model tumor invasion 

Notes

Acknowledgments

This work was supported by a grant (No. 96-2320-B-001–008) from the National Science Council and a grant (No. 95N-1003) from the National Science and Technology Program for Agricultural Biotechnology, Taiwan.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Agricultural Biotechnology Research Center, Academia SinicaTaiwanRepublic of China

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