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Gene Therapy for Antitumor Vaccination

  • Seunghee Kim-Schulze
  • Howard L. Kaufman
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 542)

Summary

Tumor immunotherapy depends on the interactions between the host, the tumor, and the immune system. Recent data suggests that priming of antigen-specific T cells alone may not be adequate for mediating regression of established tumors because of the immune inhibitory influences within the tumor microenvironment. Thus, we developed a recombinant vaccinia virus vector to express single or multiple T cell costimulatory molecules as a vector for local gene therapy in patients with malignant melanoma. This approach is feasible and generated local and systemic tumor immunity and induced objective clinical responses in patients with metastatic disease. This chapter reviews the details and major issues related to using live, replicating, recombinant poxviruses for gene delivery and antitumor vaccination within the tumor microenvironment.

Key Words

Antitumor vaccination mmunotherapy melanoma tumor antigen vaccinia virus 

Notes

Acknowledgments

We thank Dr. Bret Taback, Gail DeRaffele, RN, and Josephine Mitcham for clinical and data management support; and Dae Won Kim and Dorota Moroziewicz for technical assistance. This work was supported by National Institutes of Health (NIH) grant RO1 CA093696.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Seunghee Kim-Schulze
    • 1
  • Howard L. Kaufman
    • 1
  1. 1.The Tumor Immunology Laboratory, Division of Surgical OncologyColumbia UniversityNew YorkUSA

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