Summary
The localization pattern of the seven herpes simplex virus (HSV) DNA replication proteins is dependent upon the status of viral DNA synthesis in the infected cell. Normally, the replication proteins accumulate within replication compartments, which expand as viral DNA synthesis increases. If viral replication is blocked, either by the addition of drugs or a genetic lesion, prereplicative sites are observed. Observing the distribution of a GFP-tagged HSV replication protein can monitor the progression of viral replication. Here, we demonstrate the use of an ICP8-GFP fusion protein to observe the status of HSV replication in cultured cells by the formation of viral replication compartments.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Taylor, T.J., Knipe, D.M. (2009). The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication. In: Hicks, B.W. (eds) Viral Applications of Green Fluorescent Protein. Methods in Molecular Biology™, vol 515. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-559-6_16
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DOI: https://doi.org/10.1007/978-1-59745-559-6_16
Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-59745-559-6
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