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Real-Time PCR Assays for High-Throughput Blood Group Genotyping

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Book cover DNA and RNA Profiling in Human Blood

Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 496))

Abstract

There are multiple situations in the context of transfusion medicine where the classic serologic methods are unable to provide an adequate response, for example, recently polytransfused patients, patients with positive direct human antiglobulin tests, and hemolytic disease of the newborn. The traditional polymerase chain reaction techniques are slow and sometimes difficult to carry out and interpret. Thus there is a need for the development and validation of rapid and effective molecular methods. The genetic basis of the main alleles of the most important blood groups are known, but the frequencies vary in the different populations, thus for the genetic techniques to be efficient it is important to evaluate them, in order to adapt the molecular approaches.

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Acknowledgments

To my Supervisor, J. Eduardo Guimar aes, MD, PhD, Director of the Clinical Hematology Departments of the Oporto Faculty of Medicine and S ao Jo ao Hospital, for all the support, encouragement, and friendship during my training as a Doctor and in the carrying out of the thesis. To my Supervisor, Jill R. Storry, MSc, PhD, for her availability to orientate the thesis, for the criticism, suggestions, and knowledge shared, and for the certainty of being able to count on her support in future investigation assignments. To A. Rosa Ara’ujo, MD, recently deceased and former Director of the Transfusion Medicine and Blood Bank Department of the S ao Jo ao Hospital, for having taught me the basic fundamentals of science and investigation. To the Technical Graduates in Clinical Analyses and Public Health, F’atima Monteiro, Isabel Henriques, Elsa Meireles, Aldina Cruz, Marina Ferreira, Gilberto Tavares, and Vitor Cunha, of the Molecular Biology Centre of the Transfusion Medicine and Blood Bank Department of the S ao Jo ao Hospital, for the indispensable help in carrying out the technical aspects of the assignment.

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Authors and Affiliations

  1. Molecular Biology Center Department of Transfusion Medicine and Blood Center, Medicine Faculty of Oporto University and S. João Hospital, Portugal

    Fernando Araujo (MD, PHD)

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  1. Fernando Araujo
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Editors and Affiliations

  1. Institute of Transfusion Medicine and Immunology, Heidelberg University Medical Faculty of Mannheim, Mannheim, Germany

    Peter Bugert

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Araujo, F. (2009). Real-Time PCR Assays for High-Throughput Blood Group Genotyping. In: Bugert, P. (eds) DNA and RNA Profiling in Human Blood. METHODS IN MOLECULAR BIOLOGY™, vol 496. Humana Press. https://doi.org/10.1007/978-1-59745-553-4_3

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  • DOI: https://doi.org/10.1007/978-1-59745-553-4_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-93-0

  • Online ISBN: 978-1-59745-553-4

  • eBook Packages: Springer Protocols

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