Lithographically Defined Two- and Three-Dimensional Tissue Microarrays
Traditional methods to study normal and pathological development of tissues have been limited by difficulties in controlling experimental conditions and quantifying biological processes of interest. Here we describe methods to create microarrays of engineered tissues that enable controlled and quantitative investigations. Using soft lithography-based techniques, extracellular matrix proteins can be microcontact printed or micromolded to make two- and three-dimensional micropatterned scaffolds. The ultimate form and resulting properties of the tissue construct are dictated by the geometry of the patterned extracellular matrix components. This chapter describes elastomeric stamp fabrication, microcontact printing and micromolding of extracellular matrix proteins, cell culture in micropatterned substrata, and quantitative immunofluorescence analysis of micropatterned tissues.
Key wordsTissue engineering Microfabrication Organotypic culture Epithelial
This work was supported by grants from the NIH (CA128660 and GM083997), Susan G. Komen for the Cure (FAS0703855); and the David & Lucile Packard Foundation. C.M.N. holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund. E.W.G. was supported by post doctoral fellowships from the New Jersey Commission on Cancer Research and Susan G. Komen for the cure.