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Two-Photon Imaging of Capillary Blood Flow in Olfactory Bulb Glomeruli

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Book cover Dynamic Brain Imaging

Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 489))

Abstract

Two-photon laser scanning microscopy (TPLSM) is an efficient tool to study cerebral blood flow (CBF) and cellular activity in depth in the brain. We describe here the advantages and weaknesses of the olfactory bulb as a model to study neurovascular coupling using TPLSM. By combining intra- and extracellular recordings, TPLSM of CBF in individual capillaries, local application of drugs, we show that odor triggers odorant-specific and concentration-dependent increases in CBF. We also demonstrate that activation of neurons is required to trigger blood flow responses.

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Authors and Affiliations

  1. Laboratory of Neurophysiology; Université Paris Descartes, INSERM U603, Paris, France

    Pascale Tiret, Emmanuelle Chaigneau, Jérôme Lecoq & Serge Charpak

Authors
  1. Pascale Tiret
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  2. Emmanuelle Chaigneau
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  3. Jérôme Lecoq
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  4. Serge Charpak
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Editors and Affiliations

  1. Yale University, New Haven, CT, USA

    Fahmeed Hyder

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Tiret, P., Chaigneau, E., Lecoq, J., Charpak, S. (2009). Two-Photon Imaging of Capillary Blood Flow in Olfactory Bulb Glomeruli. In: Hyder, F. (eds) Dynamic Brain Imaging. METHODS IN MOLECULAR BIOLOGY™, vol 489. Humana Press. https://doi.org/10.1007/978-1-59745-543-5_4

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  • DOI: https://doi.org/10.1007/978-1-59745-543-5_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-74-9

  • Online ISBN: 978-1-59745-543-5

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