Modern functional neuroimaging techniques, including positron emission tomography, optical imaging of intrinsic signals, and magnetic resonance imaging (MRI) rely on a tight coupling between neural activity and cerebral blood flow (CBF) to visualize brain activity using CBF as a surrogate marker. Because the spatial and temporal resolution of neuroimaging modalities is ultimately determined by the spatial and temporal specificity of the underlying hemodynamic signals, characterization of the spatial and temporal profiles of the hemodynamic response to focal brain stimulation is of paramount importance for the correct interpretation and quantification of functional data. The ability to properly measure and quantify CBF with MRI is a major determinant of progress in our understanding of brain function. We review the dynamic arterial spin labeling (DASL) method to measure CBF and the CBF functional response with high temporal resolution.
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References
Rakic,P. (2002) Evolving concepts of cortical radial and areal specification. Prog Brain Res, 136, 265–280.
Ogawa,S., Tank,D.W., Menon,R., Ellermann,J.M., Kim,S.G., Merkle,H., Ugurbil,K. (1992) Intrinsic signal changes accompanying sensory stimulation: Functional brain mapping with magnetic resonance imaging. Proc Natl Acad Sci U S A, 89, 5951–5955.
Phelps,M.E., Mazziotta,J.C., Huang,S.C. (1982) Study of cerebral function with positron computed tomography. J Cereb Blood Flow Metab, 2, 113–162.
Lieke,E.E., Frostig,R.D., Arieli,A., Ts’o,D.Y., Hildesheim,R., Grinvald,A. (1989) Optical imaging of cortical activity: Real-time imaging using extrinsic dye-signals and high resolution imaging based on slow intrinsic-signals. Annu Rev Physiol, 51,543–559.
Villringer,A., Dirnagl,U. (1995) Coupling of brain activity and cerebral blood flow: Basis of functional neuroimaging. Cerebrovasc Brain Metab Rev, 7, 240–276.
Lauritzen,M. (2001) Relationship of spikes, synaptic activity, and local changes of cerebral blood flow. J. Cereb. Blood Flow Metab, 21, 1367–1383.
Attwell,D., Iadecola,C. (2002) The neural basis of functional brain imaging signals. Trends Neurosci, 25, 621–625.
Iadecola,C. (2004) Neurovascular regulation in the normal brain and in Alzheimer’s disease. Nat. Rev. Neurosci., 5, 347–360.
Logothetis,N.K. (2003) The underpinnings of the BOLD functional magnetic resonance imaging signal. J Neurosci, 23, 3963–3971.
Lauritzen,M. (2005) Reading vascular changes in brain imaging: Is dendritic calcium the key? Nat Rev Neurosci, 6, 77–85.
Greenberg,J.H., Hand,P., Sylvestro,A., Reivich,M. (1979) Localized metabolic-flow coupling during functional activity. Acta Neurol. Scand., 60, 12–13.
Cox,S.B., Woolsey,T.A., Rovainen,C.M. (1993) Localized dynamic changes in cortical blood flow with whisker stimulation corresponds to matched vascular and neuronal architecture of rat barrels. J. Cereb. Blood Flow Metab, 13, 899–913.
Woolsey,T.A., Rovainen,C.M., Cox,S.B., Henegar,M.H., Liang,G.E., Liu,D., Moskalenko,Y.E., Sui,J., Wei,L. (1996) Neuronal units linked to microvascular modules in cerebral cortex: Response elements for imaging the brain. Cereb Cortex, 6, 647–660.
Gerrits,R.J., Raczynski,C., Greene,A.S., Stein,E.A. (2000) Regional cerebral blood flow responses to variable frequency whisker stimulation: An autoradiographic analysis. Brain Res, 864, 205–212.
Ehler,E., Karlhuber,G., Bauer,H.C., Draeger,A. (1995) Heterogeneity of smooth muscle-associated proteins in mammalian brain microvasculature. Cell Tissue Res., 279, 393–403.
Harrison,R.V., Harel,N., Panesar,J., Mount,R.J. (2002) Blood capillary distribution correlates with hemodynamic-based functional imaging in cerebral cortex. Cereb. Cortex, 12, 225–233.
Patel,U. (1983) Non-random distribution of blood vessels in the posterior region of the rat somatosensory cortex. Brain Res,289, 65–70.
Masamoto,K., Kurachi,T., Takizawa,N., Kobayashi,H., Tanishita,K. (2004) Successive depth variations in microvascular distribution of rat somatosensory cortex. Brain Res, 995, 66–75.
Duong,T.Q., Kim,D.S., Ugurbil,K., Kim,S.G. (2001) Localized cerebral blood flow response at submillimeter columnar resolution. Proc Natl Acad Sci U S A, 98, 10904–10909.
Narayan,S.M., Santori,E.M., Toga,A.W. (1994) Mapping functional activity in rodent cortex using optical intrinsic signals. Cereb Cortex 1994 Mar-Apr;4(2):195-, 4, 195–204.
Yang,X., Hyder,F., Shulman,R.G. (1996) Activation of single whisker barrel in rat brain localized by functional magnetic resonance imaging. Proc Natl Acad Sci U S A, 93, 475–478.
Yang,X., Hyder,F., Shulman,R.G. (1997) Functional MRI BOLD signal coincides with electrical activity in the rat whisker barrels. Magn Reson Med, 38, 874–877.
Kurth,R., Villringer,K., Curio,G., Wolf,K.J., Krause,T., Repenthin,J., Schwiemann,J., Deuchert,M., Villringer,A. (2000) fMRI shows multiple somatotopic digit representations in human primary somatosensory cortex. Neuroreport, 11, 1487–1491.
Overduin,S.A., Servos,P. (2004) Distributed digit somatotopy in primary somatosensory cortex. Neuroimage, 23, 462–472.
Kida,I., Xu,F., Shulman,R.G., Hyder,F. (2002) Mapping at glomerular resolution: fMRI of rat olfactory bulb. Magn Reson Med, 48, 570–576.
Schafer,J.R., Kida,I., Xu,F., Rothman,D.L., Hyder,F. (2006) Reproducibility of odor maps by fMRI in rodents. Neuroimage, 31, 1238–1246.
Silva,A.C., Lee,S.P., Iadecola,C., Kim,S.G. (2000) Early temporal characteristics of cerebral blood flow and deoxyhemoglobin changes during somatosensory stimulation. J. Cereb. Blood Flow Metab, 20, 201–206.
Silva,A.C., Koretsky,A.P. (2002) Laminar specificity of functional MRI onset times during somatosensory stimulation in rat. Proc. Natl. Acad. Sci. U. S. A., 99, 15182–15187.
Lu,H., Patel,S., Luo,F., Li,S.J., Hillard,C.J., Ward,B.D., Hyde,J.S. (2004) Spatial correlations of laminar BOLD and CBV responses to rat whisker stimulation with neuronal activity localized by Fos expression. Magn Reson Med, 52, 1060–1068.
Bonhoeffer,T., Kim,D.S., Malonek,D., Shoham,D., Grinvald,A. (1995) Optical imaging of the layout of functional domains in area 17 and across the area 17/18 border in cat visual cortex. Eur. J. Neurosci., 7,1973–1988.
Malonek,D., Grinvald,A. (1996) Interactions between electrical activity and cortical microcirculation revealed by imaging spectroscopy: implications for functional brain mapping. Science, 272, 551–554.
Malonek,D., Dirnagl,U., Lindauer,U., Yamada,K., Kanno,I., Grinvald,A. (1997) Vascular imprints of neuronal activity: Relationships between the dynamics of cortical blood flow, oxygenation, and volume changes following sensory stimulation. Proc. Natl. Acad. Sci. U. S. A, 94, 14826–14831.
Menon,R.S., Ogawa,S., Strupp,J.P., Ugurbil,K. (1997) Ocular dominance in human V1 demonstrated by functional magnetic resonance imaging. J Neurophysiol, 77, 2780–2787.
Kim,D.S., Duong,T.Q., Kim,S.G. (2000) High-resolution mapping of iso-orientation columns by fMRI. Nat. Neurosci., 3, 164–169.
Cheng,K., Waggoner,R.A., Tanaka,K. (2001) Human ocular dominance columns as revealed by high-field functional magnetic resonance imaging. Neuron, 32, 359–374.
Goodyear,B.G., Menon,R.S. (2001) Brief visual stimulation allows mapping of ocular dominance in visual cortex using fMRI. Hum Brain Mapp, 14, 210–217.
Goodyear,B.G., Nicolle,D.A., Menon,R.S. (2002) High resolution fMRI of ocular dominance columns within the visual cortex of human amblyopes. Strabismus, 10,129–136.
Kim,S.G., Duong,T.Q. (2002) Mapping cortical columnar structures using fMRI. Physiol Behav., 77, 641–644.
Fukuda,M., Moon,C.H., Wang,P., Kim,S.G. (2006) Mapping iso-orientation columns by contrast agent-enhanced functional magnetic resonance imaging: Reproducibility, specificity, and evaluation by optical imaging of intrinsic signal. J Neurosci, 26, 11821–11832.
Sheth,S.A., Nemoto,M., Guiou,M.W., Walker,M.A., Toga,A.W. (2005) Spatiotemporal evolution of functional hemodynamic changes and their relationship to neuronal activity. J Cereb Blood Flow Metab, 25, 830–841.
Narayan,S.M., Santori,E.M., Toga,A.W. (1994) Mapping functional activity in rodent cortex using optical intrinsic signals. Cereb Cortex 1994 Mar-Apr;4(2):195-, 4, 195–204.
Narayan,S.M., Esfahani,P., Blood,A.J., Sikkens,L., Toga,A.W. (1995) Functional increases in cerebral blood volume over somatosensory cortex. J Cereb Blood Flow Metab, 15, 754–765.
Berwick,J., Martin,C., Martindale,J., Jones,M., Johnston,D., Zheng,Y., Redgrave,P., Mayhew,J. (2002) Hemodynamic response in the unanesthetized rat: Intrinsic optical imaging and spectroscopy of the barrel cortex. J. Cereb. Blood Flow Metab, 22, 670–679.
Martindale,J., Mayhew,J., Berwick,J., Jones,M., Martin,C., Johnston,D., Redgrave,P., Zheng,Y. (2003) The hemodynamic impulse response to a single neural event. J. Cereb. Blood Flow Metab, 23, 546–555.
Berwick,J., Johnston,D., Jones,M., Martindale,J., Redgrave,P., McLoughlin,N., Schiessl,I., Mayhew,J.E. (2005) Neurovascular coupling investigated with two-dimensional optical imaging spectroscopy in rat whisker barrel cortex. Eur J Neurosci., 22, 1655–1666.
Narayan,S.M., Santori,E.M., Toga,A.W. (1994) Mapping functional activity in rodent cortex using optical intrinsic signals. Cereb Cortex 1994 Mar-Apr;4(2):195-, 4, 195–204.
Friston,K.J., Holmes,A.P., Poline,J.B., Grasby,P.J., Williams,S.C., Frackowiak,R.S., Turner,R. (1995) Analysis of fMRI time-series revisited. Neuroimage., 2, 45–53.
Boynton,G.M., Engel,S.A., Glover,G.H., Heeger,D.J. (1996) Linear systems analysis of functional magnetic resonance imaging in human V1. J. Neurosci., 16, 4207–4221.
Aguirre,G.K., Zarahn,E., D’Esposito,M. (1998) The variability of human, BOLD hemodynamic responses. Neuroimage, 8, 360–369.
de Zwart,J.A., Silva,A.C., van Gelderen,P., Kellman,P., Fukunaga,M., Chu,R., Koretsky,A.P., Frank,J.A., Duyn,J.H. (2005) Temporal dynamics of the BOLD fMRI impulse response. Neuroimage., 24, 667–677.
Detre,J.A., Zhang,W., Roberts,D.A., Silva,A.C., Williams,D.S., Grandis,D.J., Koretsky,A.P., Leigh,J.S. (1994) Tissue specific perfusion imaging using arterial spin labeling. NMR Biomed, 7, 75–82.
Calamante,F., Thomas,D.L., Pell,G.S., Wiersma,J., Turner,R. (1999) Measuring cerebral blood flow using magnetic resonance imaging techniques. J. Cereb. Blood Flow Metab, 19, 701–735.
Barbier,E.L., Silva,A.C., Kim,S.G., Koretsky,A.P. (2001) Perfusion imaging using dynamic arterial spin labeling (DASL). Magn. Reson. Med., 45, 1021–1029.
Golay,X., Hendrikse,J., Lim,T.C. (2004) Perfusion imaging using arterial spin labeling. Top. Magn Reson. Imaging, 15, 10–27.
Kety,S.S. (1951) The theory and applications of inert gas exchange at the lungs and tissues. Pharmacol. Rev., 3, 1–41.
Kety,S.S. (1985) Regional cerebral blood flow: Estimation by means of nonmetabolized diffusible tracers — an overview. Semin. Nucl. Med., 15, 324–328.
Barbier,E.L., Lamalle,L., Decorps,M. (2001) Methodology of brain perfusion imaging. J Magn Reson Imaging, 13, 496–520.
Detre,J.A., Leigh,J.S., Williams,D.S., Koretsky,A.P. (1992) Perfusion imaging. Magn Reson Med, 23, 37–45.
Williams,D.S., Detre,J.A., Leigh,J.S., Koretsky,A.P. (1992) Magnetic resonance imaging of perfusion using spin inversion of arterial water. Proc Natl Acad Sci U S A, 89, 212–216.
Edelman,R.R., Siewert,B., Darby,D.G., Thangaraj,V., Nobre,A.C., Mesulam,M.M., Warach,S. (1994) Qualitative mapping of cerebral blood flow and functional localization with echo-planar MR imaging and signal targeting with alternating radio frequency. Radiology 1994 Aug;192(2):513-, 192, 513–520.
Kwong,K.K., Chesler,D.A., Weisskoff,R.M., Donahue,K.M., Davis,T.L., Ostergaard,L., Campbell,T.A., Rosen,B.R. (1995) MR perfusion studies with T1-weighted echo planar imaging. Magn. Reson. Med., 34, 878–887.
Kim,S.G. (1995) Quantification of relative cerebral blood flow change by flow-sensitive alternating inversion recovery (FAIR) technique: Application to functional mapping. Magn Reson. Med., 34, 293–301.
Wong,E.C., Buxton,R.B., Frank,L.R. (1997) Implementation of quantitative perfusion imaging techniques for functional brain mapping using pulsed arterial spin labeling. NMR Biomed., 10, 237–249.
Wong,E.C., Buxton,R.B., Frank,L.R. (1998) A theoretical and experimental comparison of continuous and pulsed arterial spin labeling techniques for quantitative perfusion imaging. Magn Reson. Med., 40, 348–355.
Barbier,E.L., Silva,A.C., Kim,H.J., Williams,D.S., Koretsky,A.P. (1999) Perfusion analysis using dynamic arterial spin labeling (DASL). Magn. Reson. Med., 41, 299–308.
Zhang,W., Williams,D.S., Detre,J.A., Koretsky,A.P. (1992) Measurement of brain perfusion by volume-localized NMR spectroscopy using inversion of arterial water spins: Accounting for transit time and cross-relaxation. Magn Reson Med, 25, 362–371.
Silva,A.C., Kim,S.G. (1999) Pseudo-continuous arterial spin labeling technique for measuring CBF dynamics with high temporal resolution. Magn. Reson. Med., 42,425–429.
Gao,J.H., Holland,S.K., Gore,J.C. (1988) Nuclear magnetic resonance signal from flowing nuclei in rapid imaging using gradient echoes. Med. Phys., 15, 809–814.
Iadecola,C. (1993) Regulation of the cerebral microcirculation during neural activity: is nitric oxide the missing link? Trends. Neurosci., 16, 206–214.
Armstrong-James,M., Fox,K., Das-Gupta,A. (1992) Flow of excitation within rat barrel cortex on striking a single vibrissa. J Neurophysiol, 68, 1345–1358.
Paiva,F.F., Tannus,A., Silva,A.C. (2007) Measurement of cerebral perfusion territories using arterial spin labelling. NMR Biomed, 20, 633–642.
Acknowledgment
This research was supported by the Intramural Research Program of the NIH, National Institute for Neurological Disorders and Stroke.
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Silva, A.C., Paiva, F.F. (2009). Dynamic Magnetic Resonance Imaging of Cerebral Blood Flow Using Arterial Spin Labeling. In: Hyder, F. (eds) Dynamic Brain Imaging. METHODS IN MOLECULAR BIOLOGY™, vol 489. Humana Press. https://doi.org/10.1007/978-1-59745-543-5_13
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