Summary
This technique is based on the sensitization of different antigens in a single nitrocellulose strip, which react when exposed to an immune serum and thereafter with the appropriate peroxidase conjugate and the corresponding substrate. Signals in those reactive spots are recorded as black squares in a negative photographic film, using a chemiluminiscent substrate or as blue spots when a precipitable colorimetric substrate is used. This technique allows the simultaneous demonstration of antigenicity of different antigens (peptides, recombinant molecules, and crude preparations), with a high sensitivity and specificity. Its major value is based on its versatility, since it is possible to rapidly evaluate and to compare various antigenic preparations and to use it for diagnosis of different infectious, allergic and autoimmune diseases, at a low cost.
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Acknowledgments
This work has been partially supported by grants N° G-2005000387 and F-2005000122 from FONACIT-Venezuela. We thank Lic. Cecilia Colmenares for technical assistance.
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Noya, O., Losada, S., Toledo, M., Alarcón de Noya, B. (2009). The Multiple Antigen Blot Assay: A Simple, Versatile and Multipurpose Immunoenzymatic Technique. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_26
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DOI: https://doi.org/10.1007/978-1-59745-542-8_26
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