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Expression, Purification, and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 517)

Summary

Toll-like receptors (TLRs) and interleukin-1 receptor (IL-1R) play crucial roles in host innate immune response against microbial infections. These receptors share a conserved cytoplasmic domain, the Toll/interleukin-1 receptor (TIR) domain, which is required for signaling through these receptors. Structural information on the TIR domains will be essential for understanding the molecular basis for signal transduction by these receptors.

Key words

Innate immunity Adaptive immunity Crystallography Protein structure and function Signal transduction Protein complexes 
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Notes

Acknowledgments

We thank Yingwu Xu and Javed Khan for their contributions to the TIR domain project. This research was supported in part by a grant from the National Institutes of Health (AI49475 to LT).

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  1. 1.Laboratory of Molecular Neurobiology and BiophysicsRockefeller UniversityNew YorkUSA
  2. 2.Department of Biological SciencesColumbia UniversityNew YorkUSA

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