Summary
Yeast is a useful model organism to study the genetic and biochemical mechanisms of aging. Genomic studies of aging in yeast have been limited, however, by traditional methodologies that require a large investment of labor and resources. In this chapter, we describe a newly-developed method for quantitatively measuring the chronological life span of each strain contained in the yeast ORF deletion collection. Our approach involves determining population survival by monitoring outgrowth kinetics using a Bioscreen C MBR shaker/incubator/plate reader. This method has accuracy comparable to traditional assays, while allowing for higher throughput and decreased variability in measurement.
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Acknowledgments
The development of the method described here was supported by a pilot project grant to MK from the University of Washington Nathan Shock Center for Excellence in the Basic Biology of Aging Grant 5P30 AG013280. CRB is supported by National Institutes of Health Training Grant 5P30 AG013280.
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Burtner, C.R., Murakami, C.J., Kaeberlein, M. (2009). A Genomic Approach to Yeast Chronological Aging. In: Stagljar, I. (eds) Yeast Functional Genomics and Proteomics. Methods in Molecular Biology, vol 548. Humana Press. https://doi.org/10.1007/978-1-59745-540-4_6
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DOI: https://doi.org/10.1007/978-1-59745-540-4_6
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