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The Roboocyte

Automated Electrophysiology Based on Xenopus Oocytes
  • Christine Leisgen
  • Mike Kuester
  • Christoph Methfessel
Part of the Methods in Molecular Biology™ book series (MIMB, volume 403)

Summary

Automated electrophysiological assays are of great importance for modern drug discovery, and various approaches have been developed into practical devices. Here, we describe the automation of two-electrode voltage-clamp (TEVC) recording from Xenopus oocytes using the Roboocyte automated workstation, jointly developed by Multi Channel Systems and Bayer Technology Services. We briefly discuss the technology, including its advantages and limitations relative to patch clamp and other TEVC systems. We provide a step-by-step description of typical operating procedures and show that the Roboocyte represents a practical and highly effective way to perform automated electrophysiology in an industrial setting.

Key Words

Xenopus oocytes mRNA expression cDNA expression two-electrode voltage clamp electrophysiology membrane receptors ion channels screening ligand gated voltage gated transporters 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Christine Leisgen
  • Mike Kuester
  • Christoph Methfessel

There are no affiliations available

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