Summary
The method described here to differentiate mouse embryonic stem (ES) cells into cardiomyocytes is adapted from Maltsev et al. and results in a high percentage of spontaneously beating cardiomyocyte-like cells. In order to determine to what extent the differentiating ES cells resemble true cardiomyocytes, the cells were electrophysiologically characterized during differentiation, using the whole-cell variant of the patch-clamp technique. Action potentials (APs) and membrane currents were recorded and analyzed off-line to determine electrophysiological changes during development.
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References
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Acknowledgments
We would like to thank C. Verhoek-Pockock and Dr. M.A. van Roon for their helpful suggestions.
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© 2007 Humana Press Inc.
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van Ginneken, A.C.G., Fijnvandraat, A.C. (2007). Electrophysiological Properties of Embryonic Stem Cells During Differentiation Into Cardiomyocyte-Like Cell Types. In: Molnar, P., Hickman, J.J. (eds) Patch-Clamp Methods and Protocols. Methods in Molecular Biology™, vol 403. Humana Press. https://doi.org/10.1007/978-1-59745-529-9_14
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DOI: https://doi.org/10.1007/978-1-59745-529-9_14
Publisher Name: Humana Press
Print ISBN: 978-1-58829-698-6
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