Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique
Control of membrane voltage and membrane current measurements are of strong interest for the study of numerous aspects of skeletal muscle physiology and pathophysiology. The silicone-clamp technique makes use of a conventional patch-clamp apparatus to achieve whole-cell voltage clamp of a restricted portion of a fully differentiated adult skeletal muscle fiber. The major part of an isolated muscle fiber is insulated from the extracellular medium with silicone grease, and the tip of a single microelectrode connected to the amplifier is then inserted within the fiber through the silicone layer. This method represents an alternative to the traditional vaseline-gap isolation and two or three microelectrode voltage-clamp techniques. This chapter reviews the main benefits of the silicone-clamp technique and provides detailed insights into its practical implementation.
Key WordsSkeletal muscle voltage clamp silicone grease mammalian muscle ion channels excitation–contraction coupling
This work was supported by the Centre National de la Recherche Scientifique, the Université Claude Bernard Lyon 1, and the Association Française contre les Myopathies.