Meiosis pp 65-76 | Cite as

Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe

  • Gerald R. Smith
Part of the Methods in Molecular Biology book series (MIMB, volume 557)


The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays of intragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.

Key words

fission yeast Schizosaccharomyces pombe meiosis intragenic recombination (gene conversion) intergenic recombination (crossing-over) spore viability tetrad analysis 



I am grateful to Sue Amundsen, Gareth Cromie, and Luther Davis for helpful comments on the manuscript. My laboratory is supported by research grants GM031693 and GM032194 from the National Institutes of Health.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Gerald R. Smith
    • 1
  1. 1.Division of Basic Sciences, Fred Hutchinson Cancer Research CenterSeattleUSA

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