Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae
Interest in crossover interference in yeast has been spurred by the discovery and characterization of mutants that alter it as well as by the development and testing of models to explain it. This chapter describes methods for detecting and for measuring interference, with emphasis on those that exploit the ability to examine all four products of individual acts of meiosis.
Key wordsNPD ratio coefficient of coincidence counting model Erlang distribution Gamma distribution Chi-square distribution
F.W.S. thanks John P. Nolan for drawing his attention to the view that the distribution of intercrossover distances in the Counting Model is aptly referred to as an Erlang distribution. Tom Petes notified us that the Web site “Stahl Lab Online Tools” mindlessly calculated fN exp values for fT obs > 2/3. It no longer does. Jette Foss provided invaluable editing of the manuscript. The Genetics Community is grateful to Richard Lowry, author of the continually improving, user-friendly web site VassarStats. Dan Graham kindly updated Stahl Lab Online Tools to reflect things we learned during the preparation of this Chapter; in doing so he caught some mistakes in our manuscript. The contribution by E.A.H. was supported by an NSF grant (DMS-0306243) to Indiana University.
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