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Meiosis pp 323-355 | Cite as

Analysis of Meiotic Recombination Products from Human Sperm

  • Liisa Kauppi
  • Celia A. May
  • Alec J. Jeffreys
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 557)

Abstract

Traditional methods for surveying meiotic recombination in humans are limited to pedigree and linkage disequilibrium analyses. We have developed assays that allow the direct detection of crossover and gene conversion molecules in batches of sperm DNA. To date, we have characterized 26 recombination hotspots by allele-specific PCR and selectively amplified recombinant DNA molecules from these regions. These analyses have revealed that meiotic crossover hotspots in humans are highly localized and flanked by DNA segments where recombination is suppressed. The centers of crossover hotspots are also active in noncrossover recombination, displaying short conversion tracts.

Key words

Allele-specific PCR haplotype sperm DNA crossover gene conversion 

Notes

Acknowledgments

Development of these methods was carried out at the University of Leicester with generous support by grants to A.J.J. from The Medical Research Council, The Louis-Jeantet Foundation and The Royal Society. L.K. was supported by the Osk. Huttunen Foundation, the Finnish Cultural Foundation, the Instrumentarium Science Foundation, and the Helsingin Sanomat Centennial Foundation.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Liisa Kauppi
    • 1
  • Celia A. May
    • 2
  • Alec J. Jeffreys
    • 3
  1. 1.Molecular Biology ProgramMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  2. 2.Department of GeneticsUniversity of LeicesterUK
  3. 3.Department of GeneticsUniversity of LeicesterUK

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