Genome-Wide High-Resolution Chromatin Immunoprecipitation of Meiotic Chromosomal Proteins in Saccharomyces cerevisiae
Cooperative actions of chromosomal proteins play critical roles in the dynamics, structural transition, segregation, and maintenance of meiotic chromosomes. A high-resolution genome-tiling array combined with a chromatin immunoprecipitation assay (ChIP-chip) is a powerful tool for uncovering the precise and dynamic distributions of various proteins along meiotic chromosomes. In this chapter, we describe a method to map the binding sites of meiotic chromosomal proteins such as Spo11, Mre11, and Rec8 using the high-resolution ChIP-chip technology. This system provides us with informative knowledge on the interplay of meiotic chromosomal proteins.
Key wordsRecombination meiosis ChIP-chip genome-tiling array DNA–protein interaction
We would like to thank all the laboratory members of Ohta laboratory and Shibata distinguished senior scientist laboratory for helpful discussion. We also thank Dr. W. Lin for critically reading this manuscript and Ms. Y. Sakuma for technical assistance. This work was supported by grants from the following sources: basic research from the Bio-oriented Technology Research Advancement Institution (to T. Shibata and K. Ohta); grants-in-aid for scientific research on priority areas from the Ministry of Education, Science, Culture, & Sports, Japan (to K. Ohta).