Meiosis pp 197-207 | Cite as

Detection of SPO11-Oligonucleotide Complexes from Mouse Testes

  • Jing Pan
  • Scott Keeney
Part of the Methods in Molecular Biology book series (MIMB, volume 557)


The SPO11 protein generates programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination. Endonucleolytic cleavage 3′ to the DSB sites releases SPO11 from DNA, leaving SPO11 covalently associated with an oligonucleotide. This chapter describes detection of the release product, SPO11-oligonucleotide complexes, from mouse testis lysates. The method for determining the size of SPO11-associated oligonucleotides is also provided.

Key words

SPO11 mouse testes terminal transferase DNA double-strand break 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jing Pan
    • 1
  • Scott Keeney
    • 2
  1. 1.Molecular Biology ProgramMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  2. 2.Howard Hughes Medical Institute and Molecular Biology Program, Memorial Sloan-Kettering Cancer CenterNew YorkUSA

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