Skip to main content

Planar Patch Clamp: Advances in Electrophysiology

  • Protocol
Potassium Channels

Summary

Ion channels have gained increased interest as therapeutic targets over recent years, since a growing number of human and animal diseases have been attributed to defects in ion channel function. Potassium channels are the largest and most diverse family of ion channels. Pharmaceutical agents such as Glibenclamide, an inhibitor of KATP channel activity which promotes insulin release, have been successfully sold on the market for many years. So far, only a small group of the known ion channels have been addressed as potential drug targets. The functional testing of drugs on these ion channels has always been the bottleneck in the development of these types of pharmaceutical compounds.

New generations of automated patch clamp screening platforms allow a higher throughput for drug testing and widen this bottleneck. Due to their planar chip design not only is a higher throughput achieved, but new applications have also become possible. One of the advantages of planar patch clamp is the possibility of perfusing the intracellular side of the membrane during a patch clamp experiment in the whole-cell configuration. Furthermore, the extracellular membrane remains accessible for compound application during the experiment.

Internal perfusion can be used not only for patch clamp experiments with cell membranes, but also for those with artificial lipid bilayers. In this chapter we describe how internal perfusion can be applied to potassium channels expressed in Jurkat cells, and to Gramicidin channels reconstituted in a lipid bilayer.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Ashcroft, F.M. (2000) Ion Channels and Disease. Academic, New York.

    Google Scholar 

  2. Neher, E. and Sakmann, B. (1976) Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 260, 799–802.

    Article  CAS  PubMed  Google Scholar 

  3. Hamill, O.P., Marty, E., Neher, E., et al. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391, 85–100.

    Article  CAS  PubMed  Google Scholar 

  4. Fertig, N., Blick, R.H., and Behrends, J.C. (2002) Whole cell patch clamp recording performed on a glass chip. Biophys J 82, 3056–3062.

    Article  CAS  PubMed  Google Scholar 

  5. Brüggeman, A., Stoelzle, S., George, M., et al. (2006) Microchip technology for automated and parallel patch-clamp recording. Small 2, 840–846.

    Article  Google Scholar 

  6. Farre, C., Stoelzle, S., Haarmann, C., George, M., Brüggemann, A., and Fertig, N. (2007) Automated ion channel screening: patch clamping made easy. Expert Opin Ther Tagerts 11, 557–565.

    Article  CAS  Google Scholar 

  7. Angelova, M.I. (2001) Liposome electro-formation. In Giant Vesicles (Luisi, P.L. and Walde, P. Ed.). Wiley, Chichester, pp. 27–36.

    Google Scholar 

  8. Neher, E. (1995) Voltage offsets in patch-clamp experiments. In Single-Channel Recording (Sakmann, B. and Neher, E. Eds.). Plenum, New York, pp. 147–153.

    Google Scholar 

  9. Hille, B. (1992) Ionic Channels of Excitable Membranes. Sinauer Associates, Sunderland, MA, p. 89.

    Google Scholar 

Download references

Acknowledgments

The Authors would like to thank Priv. Doz. Dr. Guiscard Seebohm for the development of the first prototype of the internal perfusion. We acknowledge financial support from the European Union within the framework of the Marie Curie Training Network 019335 “Translocation” and from the Bundesministerium fuer Bildung und Forschung (BMBF 13N9110).

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Humana Press, a part of Springer Science + Business Media

About this protocol

Cite this protocol

Brüggemann, A. et al. (2008). Planar Patch Clamp: Advances in Electrophysiology. In: Lippiat, J.D. (eds) Potassium Channels. Methods in Molecular Biology, vol 491. Humana Press. https://doi.org/10.1007/978-1-59745-526-8_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-59745-526-8_13

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-65-7

  • Online ISBN: 978-1-59745-526-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics