Summary
The array CGH technique (array comparative genome hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. Here, we present validated protocols using in-house spotted oligonucleotide libraries for array CGH. This oligo array CGH platform yields reproducible results and is capable of detecting single copy gains, multicopy amplifications as well as homozygous and heterozygous deletions with high resolution. The protocol allows as little as 300 ng of input DNA, which makes the procedure valuable for small clinical samples and is also functional for DNA samples obtained from archival tissues.
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Reference
van den IJssel, P., Tijssen, M., Chin, S. F., et al. (2005) Human and mouse oligonucleotide-based array CGH. Nucleic Acids Res. 33, e192.
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© 2007 Humana Press Inc.
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van den IJssel, P., Ylstra, B. (2007). Oligonucleotide Array Comparative Genomic Hybridization. In: Bergman, N.H. (eds) Comparative Genomics. Methods In Molecular Biology™, vol 396. Humana Press. https://doi.org/10.1007/978-1-59745-515-2_14
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DOI: https://doi.org/10.1007/978-1-59745-515-2_14
Publisher Name: Humana Press
Print ISBN: 978-1-934115-37-4
Online ISBN: 978-1-59745-515-2
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