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Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex® xMAP™ Suspension Array

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Book cover Salmonella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 394))

Abstract

We describe a suspension array hybridization assay for rapid detection and identification of Salmonella and other bacterial pathogens using Luminex® xMAP™ technology. The Luminex xMAP system allows simultaneous detection of up to 100 different targets in a single multiplexed reaction. Included in the method are the procedures for (1) design of species-specific oligonucleotide capture probes and PCR amplification primers, (2) coupling oligonucleotide capture probes to carboxylated microspheres, (3) hybridization of coupled microspheres to oligonucleotide targets, (4) production of targets from DNA samples by PCR amplification, and (5) detection of PCR-amplified targets by direct hybridization to probe-coupled microspheres. The Luminex xMAP suspension array hybridization assay is rapid, requires few sample manipulations, and provides adequate sensitivity and specificity to detect and differentiate Salmonella and nine other test organisms through direct detection of species-specific DNA sequences.

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Author information

Authors and Affiliations

  1. Luminex Corporation, Austin, TX

    Sherry A. Dunbar & James W. Jacobson

Authors
  1. Sherry A. Dunbar
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  2. James W. Jacobson
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Editor information

Editors and Affiliations

  1. Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO, USA

    Heide Schatten

  2. Cancer Research Center, Columbia, MO, USA

    Abraham Eisenstark

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© 2007 Humana Press Inc., Totowa, NJ

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Dunbar, S.A., Jacobson, J.W. (2007). Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex® xMAP™ Suspension Array. In: Schatten, H., Eisenstark, A. (eds) Salmonella. Methods in Molecular Biology, vol 394. Humana Press. https://doi.org/10.1007/978-1-59745-512-1_1

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  • DOI: https://doi.org/10.1007/978-1-59745-512-1_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-619-1

  • Online ISBN: 978-1-59745-512-1

  • eBook Packages: Springer Protocols

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