Brain on a Chip: A Method to Detect Novel Neuroprotective Candidate Targets

  • Yang Tang
  • Myriam Bernaudin
Part of the Methods in Molecular Biology book series (MIMB, volume 399)


The search of potential novel therapeutical targets for neuroprotection has been widely intensified since the usefulness of microarray techniques. Indeed, this recent technology (also called Gene chip) provides a powerful tool to examine gene expression changes of thousands of genes at the same time, on a single chip in the brain. Arrays can paint a picture or “profile” (gene profiling, gene expression patterns) of which genes in the genome are active in a particular cell type and under a particular condition. In this chapter, we will describe the methods to perform microarrays and analyze the following data using GeneChip® technology (Affymetrix, Inc., Santa Clara, CA, USA), to identify, for example, potential brain neuroprotective targets. Moreover, step-by-step explanations of software operation will be provided. Finally, methods are presented to validate the gene expression changes revealed from the microarray analyses.

Key Words

Chip microarray brain neuroprotection genomic 


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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Yang Tang
    • 1
  • Myriam Bernaudin
    • 2
  1. 1.Department of NeurologyUniversity of Minnesota Medical SchoolMinneapolis
  2. 2.UMR-CNRS 6185, Centre Cyceron, Neurodégénérescence: Modèles et Stratégies ThérapeutiquesUniversité de CaenCaenFrance

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