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Methods to Study the Interactions of the Dynein Light Chains and Intermediate Chains

  • Kevin W.-H. Lo
  • K. Kevin Pfister
Part of the Methods in Molecular Biology™ book series (MIMB, volume 392)

Abstract

The cargo-binding domain of the cytoplasmic dynein complex consists of an intermediate chain, a light-intermediate chain, and three families of light chains. These five subunits form the base of the dynein complex. Variations in the composition and interactions of these subunits play an important role for selecting a particular cargo and regulating dynein function. By using several complementary binding methods, we have investigated the protein-protein interaction in the cargo-binding domain of the cytoplasmic dynein.

Key Words

Cytoplasmic dynein motor protein microtubule cytoskeleton intermediate chain light chain protein-protein interaction 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Kevin W.-H. Lo
    • 1
  • K. Kevin Pfister
    • 1
  1. 1.Department of Cell BiologyUniversity of Virginia School of MedicineCharlottesville

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