Periodic Assembly of Nanospecies on Repetitive DNA Sequences Generated on Gold Nanoparticles by Rolling Circle Amplification
- 1.2k Downloads
Periodical assembly of nanospecies is desirable for the construction of nanodevices. We provide a protocol for the preparation of a gold nanoparticle (AuNP)/DNA scaffold on which nanospecies can be assembled in a periodical manner. AuNP/DNA scaffold is prepared by growing long single-stranded DNA (ssDNA) molecules (typically hundreds of nanometers to a few microns in length) on AuNPs via rolling circle amplification (RCA). Since these long ssDNA molecules contain many repetitive sequence units, complementary DNA-attached nanospecies can be assembled through specific hybridization in a controllable and periodical manner.
Key WordsDNA DNA amplification enzymatic manipulation gold nanotechnology rolling circle amplification self-assembly.
This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC); SENTINEL: Canadian Network for the Development and Use of Bioactive Paper; and the Canadian Institutes for Health Research (CIHR). We wish to thank Dr. Yan Gao for help with the AFM and Dr. Srinivas A. Kandadai for the helpful discussion. Y.L. holds a Canada Research Chair.
- 1.Katov NA. (2006) Nanoparticle Assemblies and Superstructures. CRC Press, Taylor and Francis, Boca Raton, FL.Google Scholar
- 2.Zhang J, Wang Z, Liu J, Chen S, Liu G. (2003) Self-Assembled Nanostructures. Kluwer Academic/Plenum, New York.Google Scholar
- 14.Rickwood D, Hames BD. (1990) Gel Electrophoresis of Nucleic Acids: A Practical Approach. IRL Press, Oxford, NY.Google Scholar
- 15.Sambrook J, Russell DW. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar