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Synthesis and Primary Characterization of Self-Assembled Peptide-Based Hydrogels

  • Radhika P. Nagarkar
  • Joel P. Schneider
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 474)

Summary

Hydrogels based on peptide self-assembly form an important class of biomaterials that find application in tissue engineering and drug delivery. It is essential to prepare peptides with high purity to achieve batch-to-batch consistency affording hydrogels with reproducible properties. Automated solid-phase peptide synthesis coupled with optimized Fmoc (9-fluorenylmethoxy-carbonyl) chemistry to obtain peptides in high yield and purity is discussed. Details of isolating a desired peptide from crude synthetic mixtures and assessment of the peptide's final purity by high-performance liquid chromatography and mass spectrometry are provided. Beyond the practical importance of synthesis and primary characterization, techniques used to investigate the properties of hydrogels are briefly discussed.

Key Words Biomaterial HPLC hydrogel peptide self-assembly solid-phase peptide synthesis 

Notes

Acknowledgments

We acknowledge the National Institutes of Health grant R01 DE016386-01. We also thank Lisa A. Haines-Butterick for optimization of the synthesizer chemistry and her helpful discussions for this chapter as well as Karthikan Rajagopal for performing the MAX3 studies.

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Radhika P. Nagarkar
    • 1
  • Joel P. Schneider
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of DelawareNewark

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