Abstract
Abscisic acid (ABA) plays a number of key roles in the growth, development, and stress response of plants. For example, it is vital to a plant’s response to drought stress, and is the signalling molecule responsible for closure of the stomata in order to promote water conservation. The hormone is rapidly turned over in plant tissue, mainly by oxidation or conjugation. Accurate and sensitive quantification of ABA and its metabolites has made a significant contribution to the knowledge of the role of this hormone, and also of its relationship to the induction of numerous ABA-induced genes in plants. High-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has become an essential technique for the analysis and quantification of these compounds.
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References
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Acknowledgments
This method has been developed by a team of PBI-NRC researchers: Sue Abrams, Steve Ambrose, Vera Čekić, Sheila Chiwocha, Allan Feurtado, Pearla Gai, Marek Galka, Patrycja Galka, Daiqing Huang, Monika Lafond, Ken Nelson, Stacey Owen, Andrew Ross, Irina Zaharia, and Rong Zhou. The authors thank Irina Zaharia and Andrew Ross for critical reading of the manuscript and acknowledge the National Research Council of Canada’s Genomics and Health Initiative (GHI) and Crops for Enhanced Human Health (CEHH) programs for funding. This contribution is NRCC no. 44848.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Owen, S.J., Abrams, S.R. (2009). Measurement of Plant Hormones by Liquid Chromatography–Mass Spectrometry. In: Cutler, S., Bonetta, D. (eds) Plant Hormones. Methods in Molecular Biology, vol 495. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-477-3_4
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DOI: https://doi.org/10.1007/978-1-59745-477-3_4
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