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Neutrophil Methods and Protocols pp 15–20Cite as

Isolation of Human Neutrophils From Venous Blood

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 412)

Abstract

Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies

Key Words

  • Granulocytes
  • mononuclear cells
  • Hypaque-Ficoll
  • dextran sedimentation

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  • DOI: 10.1007/978-1-59745-467-4_2
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References

  1. Böyum, A. (1968) Isolation of mononuclear cells and granulocytes from human blood. Scan. J. Clin. Lab. Invest. 21, 77–89.

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  2. Skoog, W. A. and Beck, W. S. (1956) Studies on the fibrinogen, dextran, and phytohemagglutinin methods of isolating leukocytes. Blood 11, 436–454.

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Author information

Authors and Affiliations

  1. Inflammation Program and Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Affairs Medical Center, Coralville, Iowa City, IA

    William M. Nauseef

Authors
  1. William M. Nauseef
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Editor information

Editors and Affiliations

  1. Deparment of Veterinary Molecular Biology, Montana State University, Bozeman, MT

    Mark T. Quinn

  2. Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT

    Frank R. DeLeo

  3. Departments of Immunology and Cell Biology, The Scripps Institute, La Jolla, CA

    Gary M. Bokoch

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© 2007 Humana Press Inc., Totowa, NJ

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Nauseef, W.M. (2007). Isolation of Human Neutrophils From Venous Blood. In: Quinn, M.T., DeLeo, F.R., Bokoch, G.M. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology™, vol 412. Humana Press. https://doi.org/10.1007/978-1-59745-467-4_2

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  • DOI: https://doi.org/10.1007/978-1-59745-467-4_2

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-788-4

  • Online ISBN: 978-1-59745-467-4

  • eBook Packages: Springer Protocols