Abstract
Transcriptome analyses of single- and multicellular organisms have changed fundamental understanding of biological and pathological processes across multiple scientific disciplines. Over the past 5 yr, studies of polymorphonuclear leukocyte (or neutrophil) (PMN) gene expression on a global scale have provided new insight into the molecular processes that promote resolution of infections in humans. Herein, we present methods to analyze gene expression in human neutrophils using Affymetrix oligonucleotide microarrays, which include isolation of high-quality RNA, generation and labeling of cRNA, and GeneChip hybridization and scanning. Notably, the procedures utilize commercially available reagents and materials and thus represent a standardized approach for evaluating PMN transcript levels.
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© 2007 Humana Press Inc., Totowa, NJ
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Kobayashi, S.D., Sturdevant, D.E., DeLeo, F.R. (2007). Genome-Scale Transcript Analyses in Human Neutrophils. In: Quinn, M.T., DeLeo, F.R., Bokoch, G.M. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology™, vol 412. Humana Press. https://doi.org/10.1007/978-1-59745-467-4_27
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DOI: https://doi.org/10.1007/978-1-59745-467-4_27
Publisher Name: Humana Press
Print ISBN: 978-1-58829-788-4
Online ISBN: 978-1-59745-467-4
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