Isolation of Human Neutrophils From Venous Blood

  • William M. Nauseef
Part of the Methods in Molecular Biology™ book series (MIMB, volume 412)


Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies

Key Words

Granulocytes mononuclear cells Hypaque-Ficoll dextran sedimentation 


  1. 1.
    Böyum, A. (1968) Isolation of mononuclear cells and granulocytes from human blood. Scan. J. Clin. Lab. Invest. 21, 77–89.CrossRefGoogle Scholar
  2. 2.
    Skoog, W. A. and Beck, W. S. (1956) Studies on the fibrinogen, dextran, and phytohemagglutinin methods of isolating leukocytes. Blood 11, 436–454.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • William M. Nauseef
    • 1
  1. 1.Inflammation Program and Department of Medicine, Roy J. and Lucille A. Carver College of MedicineUniversity of Iowa and Veterans Affairs Medical CenterCoralville, Iowa City

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