Abstract
This valuable and unique book contains a compendium of methods and reviews that does much more than allow one to study the biology of neutrophils. What makes this collection of contributions so special is that it highlights and facilitates using the neutrophil as a simple, pure, single primary cell suspension model to study a remarkable array of generalized cellular functions (ameboid cellular motility, chemotaxis, adhesion, phagocytosis, degranulation, oxygen radical production, apoptosis), biochemical pathways (G protein-coupled receptor function and regulation, ion channel function, calcium transients, phosphorylation events, actin regulation, adhesion molecule regulation), as well as specialized functions and molecules important to host defense against infection, the mediation and resolution of inflammation, and the chemokine mediated modulation of acquired immunity (see Fig. 1). As I note the array of chapter topics, it evokes some of the past history of inquiry into how the neutrophil functions and how we evolved into the current widespread use of the neutrophil as a convenient model system for studying so many types of cellular processes and biochemical pathways.
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Hoebe, K., Janssen, E., and Beutler, B. (2004) The interface between innate and adaptive immunity. Nat. Immunol. 5, 971–974.
Babior, B. M., Kipnes, R. S., and Curnutte, J. T. (1973) Biological defense mechanisms: production by leukocytes of superoxide, a potential bactericidal agent. J. Clin. Invest. 52, 741–744.
Klebanoff, S. J. (1967) Iodination of bacteria: a bactericidal mechanism. J. Exp. Med. 126, 1063–1078.
Lehrer, R. I., Hanifin, J., and Cline, M. J. (1969) Defective bactericidal activity in myeloperoxidase-deficient human neutrophils. Nature 223, 78–79.
Bainton, D. F., Ullyot, J. L., and Farquhar, M. G. (1971) The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. J. Exp. Med. 134, 907–934.
Bainton, D. F. and Farquhar, M. G. (1968) Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. I. Histochemical staining of bone marrow smears. J. Cell Biol. 39, 286–298.
Borregaard, N. and Cowland, J. B. (1997) Granules of the human neutrophilic polymorphonuclear leukocyte. Blood 89, 3503–3521.
Segal, A. W. and Abo, A. (1993) The biochemical basis of the NADPH oxidase of phagocytes. Trends Biochem. Sci. 18, 43–47.
Babior, B. M. (1999) NADPH oxidase: an update. Blood 93, 1464–1476.
Clark, R. A. (1990) The human neutrophil respiratory burst oxidase. J. Infect. Dis. 161, 1140–1147.
Zigmond, S. H. (1978) Chemotaxis by polymorphonuclear leukocytes. J. Cell. Biol. 77, 269–287.
Southwick, F. S. and Stossel, T. P. (1983) Contractile proteins in leukocyte function. Semin. Hematol. 20, 305–321.
Fliedner, T. M., Cronkite, E. P., and Robertson, J. S. (1964) Granulocytopoiesis. I. Senescence and random loss of neutrophilic granulocytes in human beings. Blood 24, 402–414.
Athens, J. W., Haab, O. P., Raab, S. O., et al. (1961) Leukokinetic studies. IV. The total blood, circulating and marginal granulocyte pools and the granulocyte turnover rate in normal subjects. J. Clin. Invest. 40, 989–995.
Rossi, F. and Zatti, M. (1964) Changes in the metabolic pattern of polymorphonuclear leukocytes during phagocytosis. Br. J. Exp. Pathol. 45, 548–559.
Nauseef, W. M. and Clark, R. A. (2000) Granulocytic phagocytes, in Basic Principles in the Diagnosis and Management of Infectious Diseases, 5th Ed. (Mandell, G. L., Bennett, J. P., and Dolin, R., eds.), Churchill Livingstone: New York, pp. 89–112.
Kobayashi, S. D., Voyich, J. M., Buhl, C. L., Stahl, R. M., and DeLeo, F. R. (2002) Global changes in gene expression by human polymorphonuclear leukocytes during receptor-mediated phagocytosis: cell fate is regulated at the level of gene expression. Proc. Natl. Acad. Sci. USA 99, 6901–6906.
Theilgaard-Mönch, K., Knudsen, S., Follin, P., and Borregaard, N. (2004) The transcriptional activation program of human neutrophils in skin lesions supports their important role in wound healing. J. Immunol. 172, 7684–7693.
Zhang, X. Q., Kluger, Y., Nakayama, Y., et al. (2004) Gene expression in mature neutrophils: early responses to inflammatory stimuli. J. Leukoc. Biol. 75, 358–372.
Strieter, R. M., Kasahara, K., Allen, R. M., et al. (1992) Cytokine-induced neutrophil-derived interleukin-8. Am. J. Pathol. 141, 397–407.
Schiffmann, E., Corcoran, B. A., and Wahl, S. M. (1975) N-formylmethionyl peptides as chemoattractants for leucocytes. Proc. Natl. Acad. Sci. USA 72, 1059–1062.
Snyderman, R. and Goetzl, E. J. (1981) Molecular and cellular mechanisms of leukocyte chemotaxis. Science 213, 830–835.
Allen, R. A., Jesaitis, A. J., and Cochrane, C. G. (1990) The N-formyl peptide receptor, in Cellular and Molecular Mechanisms of Inflammation: Receptors of Inflammatory Cells, Structure-Function Relationships (Cochrane, C. G. and Gimbrone, M. A., eds.), Academic: San Diego, pp. 83–112.
O’Flaherty, J. T., Showell, H. J., and Ward, P. A. (1977) Influence of extracellular Ca2+ and Mg2+ on chemotactic factor-induced neutrophil aggregation. Inflammation 2, 265–276.
Serhan, C. N., Broekman, M. J., Korchak, H. M., Smolen, J. E., Marcus, A. J., and Weissmann, G. (1983) Changes in phosphatidylinositol and phosphatidic acid in stimulated human neutrophils. Relationship to calcium mobilization, aggregation and superoxide radical generation. Biochim. Biophys. Acta 762, 420–428.
McPhail, L. C., Clayton, C. C., and Snyderman, R. (1984) A potential second messenger role for unsaturated fatty acids: Activation of Ca++-dependant protein kinase. Science 224, 622–625.
Aharoni, I. and Pick, E. (1990) Activation of the superoxide-generating NADPH oxidase of macrophages by sodium dodecyl sulfate in a soluble cell-free system: evidence for involvement of a G protein. J. Leukoc. Biol. 48, 107–115.
Quinn, M. T., Parkos, C. A., Walker, L., Orkin, S. H., Dinauer, M. C., and Jesaitis, A. J. (1989) Association of a ras-related protein with cytochrome b of human neutrophils. Nature 342, 198–200.
Abo, A., Pick, E., Hall, A., Totty, N., Teahan, C. G., and Segal, A. W. (1991) Activation of the NADPH oxidase involves the small GTP-binding Protein p21rac1. Nature 353, 668–670.
Knaus, U. G., Heyworth, P. G., Evans, T., Curnutte, J. T., and Bokoch, G. M. (1991) Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac2. Science 254, 1512–1515.
Serhan, C. N. and Savill, J. (2005) Resolution of inflammation: the beginning programs the end. Nat. Immunol. 6, 1191–1197.
Savill, J. S., Wyllie, A. H., Henson, J. E., Walport, M. J., Henson, P. M., and Haslett, C. (1989) Macrophage phagocytosis of aging neutrophils in inflammation. Programmed cell death in the neutrophil leads to its recognition by macrophages. J. Clin. Invest 83, 865–875.
Whyte, M. K., Meagher, L. C., MacDermot, J., and Haslett, C. (1993) Impairment of function in aging neutrophils is associated with apoptosis. J. Immunol. 150, 5124–5134.
DeLeo, F. R. (2004) Modulation of phagocyte apoptosis by bacterial pathogens. Apoptosis 9, 399–413.
Tobias, J. D. and Schleien, C. (1991) Granulocyte transfusions—a review for the intensive care physician. Anaesth. Intensive Care 19, 512–520.
Froland, S. S. (1984) Bacterial infections in the compromised host. Scand. J. Infect. Dis. Suppl 43, 7–16.
Bodey, G. P., Buckley, M., Sathe, Y. S., and Freireich, E. J. (1966) Quantitative relationships between circulating leukocytes and infection in patients with acute leukemia. Ann. Intern. Med. 64, 328–340.
Dale, D. C., Guerry, D., Wewerka, J. R., Bull, J. M., and Chusid, M. J. (1979) Chronic neutropenia. Medicine (Baltimore) 58, 128–144.
Kobayashi, S. D., Voyich, J. M., Braughton, K. R., et al. (2004) Gene expression profiling provides insight into the pathophysiology of chronic granulomatous disease. J. Immunol. 172, 636–643.
Bunting, M., Harris, E. S., McIntyre, T. M., Prescott, S. M., and Zimmerman, G. A. (2002) Leukocyte adhesion deficiency syndromes: adhesion and tethering defects involving beta 2 integrins and selectin ligands. Curr. Opin. Hematol. 9, 30–35.
Finkel, T. and Holbrook, N. J. (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408, 239–247.
Rahman, I., Biswas, S. K., and Kode, A. (2006) Oxidant and antioxidant balance in the airways and airway diseases. Eur. J. Pharmacol. 533, 222–239.
Temple, M. D., Perrone, G. G., and Dawes, I. W. (2005) Complex cellular responses to reactive oxygen species. Trends Cell Biol. 15, 319–326.
Weiss, S. J. (1989) Tissue destruction by neutrophils. N. Engl. J. Med. 320, 365–376.
Altieri, D. C. (1995) Proteases and protease receptors in modulation of leukocyte effector functions. J. Leukoc. Biol. 58, 120–127.
Zaidi, S. H., You, X. M., Ciura, S., Husain, M., and Rabinovitch, M. (2002) Overexpression of the serine elastase inhibitor elafin protects transgenic mice from hypoxic pulmonary hypertension. Circulation 105, 516–521.
Zeiher, B. G., Matsuoka, S., Kawabata, K., and Repine, J. E. (2002) Neutrophil elastase and acute lung injury: prospects for sivelestat and other neutrophil elastase inhibitors as therapeutics. Crit. Care Med. 30, S281–S287.
Ganz, T. (2004) Antimicrobial polypeptides. J. Leukoc. Biol. 75, 34–38.
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Malech, H.L. (2007). The Role of Neutrophils in the Immune System. In: Quinn, M.T., DeLeo, F.R., Bokoch, G.M. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology™, vol 412. Humana Press. https://doi.org/10.1007/978-1-59745-467-4_1
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DOI: https://doi.org/10.1007/978-1-59745-467-4_1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-788-4
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