Study of the lysosomal protein transport system has been facilitated through dissection of the analogous vacuolar protein sorting (VPS) pathway in Saccharomyces cerevisiae. Resident enzymes of the yeast vacuole are synthesized as inactive precursors and are cleaved to their mature forms upon delivery to this compartment. Quantitative assessment of this delivery can be achieved through the use of pulse-chase experiments monitoring the cleavage of zymogens to their mature forms. The experimental procedures for analysis of carboxypeptidase Y (CPY) and carboxypeptidase S (CPS) maturation are described.
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Davies, B.A., Carney, D.S., Horazdovsky, B.F. (2007). Evaluating Yeast Biosynthetic Vacuolar Transport. In: van der Giezen, M. (eds) Protein Targeting Protocols. Methods in Molecular Biology™, vol 390. Humana Press. https://doi.org/10.1007/978-1-59745-466-7_22
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DOI: https://doi.org/10.1007/978-1-59745-466-7_22
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