Abstract
This chapter describes the different methods used for localization of proteins and organelles in Pichia pastoris. A series of plasmids and a modified immunofluorescence protocol for localization and co-localization of proteins and organelles are described. Also included are protocols for the labeling of different subcellular organelles with vital stains.
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© 2007 Humana Press Inc., Totowa, NJ
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Farre, JC., Shirahama-Noda, K., Zhang, L., Booher, K., Subramani, S. (2007). Localization of Proteins and Organelles Using Fluorescence Microscopy. In: Cregg, J.M. (eds) Pichia Protocols. Methods in Molecular Biology, vol 389. Humana Press. https://doi.org/10.1007/978-1-59745-456-8_17
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DOI: https://doi.org/10.1007/978-1-59745-456-8_17
Publisher Name: Humana Press
Print ISBN: 978-1-58829-429-6
Online ISBN: 978-1-59745-456-8
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