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Collection and Preparation of Rodent Tissue Samples for Histopathological and Molecular Studies in Carcinogenesis

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Inflammation and Cancer

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 511))

Summary

Histology, as a mean of tissue visualization on a cellular level, is a fundamental tool in the study of cancer. The need for simultaneous delivery of quality histological material for pathological evaluation and subsequent genomic and proteomic studies, however, requires modification of traditional practices to include rapid isolation and stabilization of target tissue to preserve molecular integrity. Informative molecular analysis depends on the integrity of target molecules (RNA, DNA, and proteins) in the tissue during and after its collection. A reliable systematic approach to routine and genomic/proteomic sample collection and preparation presented is supported by detailed protocols.

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Acknowledgments

The authors would like to thank Dr. Lawrence R Sternberg, Head of Histotechnology Laboratory of NCI-Frederick, for his valuable advice and manuscript review, and staff of Histotechnology Laboratory for technical assistance and encouragement. This work was funded by NCI Contract N01-CO-12400.

Author information

Authors and Affiliations

  1. Histotechnology Laboratory, NCI-Frederick, SAIC-Frederick, Frederick, MD, USA

    Yelena Golubeva

  2. Department of Histopathology, Biopolis Shared Facilities (BSF), Proteos, Singapore

    Keith Rogers

Authors
  1. Yelena Golubeva
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  2. Keith Rogers
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Editor information

Editors and Affiliations

  1. National Cancer Institute at Frederick, SAIC-Frederick, Inc., Frederick, MD, USA

    Serguei V. Kozlov

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Golubeva, Y., Rogers, K. (2009). Collection and Preparation of Rodent Tissue Samples for Histopathological and Molecular Studies in Carcinogenesis. In: Kozlov, S.V. (eds) Inflammation and Cancer. Methods in Molecular Biology™, vol 511. Humana Press. https://doi.org/10.1007/978-1-59745-447-6_1

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  • DOI: https://doi.org/10.1007/978-1-59745-447-6_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-14-5

  • Online ISBN: 978-1-59745-447-6

  • eBook Packages: Springer Protocols

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