Skip to main content

Analysis of Microtubule-Mediated Intracellular Viral Transport

  • Protocol
Microtubule Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 137))

Abstract

Host microtubules and motor proteins are crucial to the intracellular transport of a number of viruses. Disruption of microtubules or suppression of motor functions can remarkably inhibit the movement of viruses in host cells. It is now known that incoming viruses use motor proteins to travel along microtubules from the plasma membrane to the nuclear or perinuclear replication site, whereas progeny viruses depend on microtubules and motors to move from the assembly site to the cell periphery. Here, we describe several major methods for analyzing microtubule-mediated intracellular viral transport, using adenovirus as an example.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Smith, G. A. and Enquist, L. W. (2002) Break ins and break outs: viral interactions with cytoskeleton of mammalian cells. Annu. Rev. Cell Dev. Biol. 18, 135–161.

    Article  CAS  PubMed  Google Scholar 

  2. Sodeik, B., Ebersold, M. W., and Helenius, A. (1997) Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus. J. Cell Biol. 136, 1007–1021.

    Article  CAS  PubMed  Google Scholar 

  3. Sodeik, B. (2002) Unchain my heart, baby let me go—the entry and intracellular transport of HIV. J. Cell Biol. 159, 393–395.

    Article  CAS  PubMed  Google Scholar 

  4. Suomalainen, M., Nakano, M. Y., Boucke, K., Keller, S., Stidwill, R. P., and Greber, U. F. (1999) Microtubule-dependent minus and plus end-directed motilities are competing processes for nuclear targeting of adenovirus. J. Cell Biol. 144, 657–672.

    Article  CAS  PubMed  Google Scholar 

  5. Dohner, K., Nagel, C. H., and Sodeik B. (2005) Viral stop-and-go along microtubules: taking a ride with dynein and kinesins. Trends Microbiol. 13, 320–327.

    Article  PubMed  Google Scholar 

  6. Döhner, K., Wolfstein, A., Prank, U., et al. (2002) Function of dynein and dynactin in herpes simplex virus capsid transport. Mol. Biol. Cell. 13, 2795–2809.

    Article  PubMed  Google Scholar 

  7. Bartlett, J. S., Wilcher, R., and Samulski, R. J. (2000) Infectious entry pathway of adeno-associated virus and adeno-associated virus vectors J. Virol. 74, 2777–2785.

    Article  CAS  PubMed  Google Scholar 

  8. Bearer E. L., Breakefield, X. O., Schuback, D., Reese, T. S., and LaVail, J. H. (2000) Retrograde axonal transport of herpes simplex virus: evidence for a single mechanism and a role for tegument. Proc. Natl. Acad. Sci. USA 97, 8146–8150.

    Article  CAS  PubMed  Google Scholar 

  9. Ward, B. M. and Moss, B. (2001) Visualization of intracellular movement of vaccinia virus virions containing a green fluorescent protein-B5R membrane protein chimera J. Virol. 75, 4802–4813.

    Article  CAS  PubMed  Google Scholar 

  10. Glotzer, J. B., Michou, A., Baker, A., Saltik, M., and Cotton, M. (2001) Microtubule-independent motility and nuclear targeting of adenoviruses with fluorescently labeled genomes. J. Virol. 75, 2421–2434.

    Article  CAS  PubMed  Google Scholar 

  11. Seisenberger, G., Ried, M. U., Endreb, T., Buning, H., Hallek, M., and Brauchle, C. (2001) Real-time single-molecule imaging of the infection pathway of an adeno-associated virus. Science 294, 1929–1932.

    Article  CAS  PubMed  Google Scholar 

  12. Mabit, H., Nakano, M. Y., Prank, U., et al. (2002) Intact microtubules support adenovirus and herpes simplex virus infections. J. Virol. 76, 9962–9971.

    Article  CAS  PubMed  Google Scholar 

  13. Valetti, C., Wetzel, D. M., Schrader, M., et al. (1999) Role of dynactin in endocytic traffic: effects of dynamitin overexpression and colocalization with CLIP-170. Mol. Biol. Cell 10, 4107–4120.

    CAS  PubMed  Google Scholar 

  14. Kelkar, S. A., Pfister, K. K., Crystal, R. G., and Leopold, P. L. (2004) cytoplasmic dynein mediates adenovirus binding to microtubules. J. Virol. 78, 10,122–10,132.

    Article  CAS  PubMed  Google Scholar 

  15. Lawrence, W. C. and Ginsberg, H. S. (1967) Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid. J. Virol. 1, 851–867.

    CAS  PubMed  Google Scholar 

  16. Greber, U. F., Willetts, M., Webster, P., and Helenius, A. (1993) Stepwise dismantling of adenovirus 2 during entry into cells. Cell 57, 477–486.

    Article  Google Scholar 

  17. Greber, U. F., Suomalainen, M., Stidwill, R. P., Boucke, K., Ebersold, M. W., and Helenius, A. (1997) The role of the nuclear pore complex in adenovirus DNA entry. EMBO J. 16, 5998–6007.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Humana Press Inc.

About this protocol

Cite this protocol

Liu, C., Liu, M., Zhou, J. (2007). Analysis of Microtubule-Mediated Intracellular Viral Transport. In: Zhou, J. (eds) Microtubule Protocols. Methods in Molecular Medicine™, vol 137. Humana Press. https://doi.org/10.1007/978-1-59745-442-1_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-59745-442-1_12

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-642-9

  • Online ISBN: 978-1-59745-442-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics