Abstract
Since RNA is believed to be the most vulnerable molecular component of unfixed tissue, preserved RNA integrity can be used as a general quality indicator in fresh frozen tissue biobanks. As the size of samples and biopsies often is small, in the range of millimeters or milligrams, it is important to implement quality control procedures adapted to minute the amounts of tissue. To this end, we here describe RNA extraction from one or a few frozen tissue sections and subsequent analysis of structural RNA integrity by microcapillary gel electrophoresis.
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Acknowledgments
The skillful assistance of Karolina Edlund is gratefully acknowledged. The Fresh Tissue Biobank project at the Department of Pathology, Uppsala University Hospital was supported by the Swedish National Biobank Platform funded by Wallenberg Consortium North and Swegene.
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Botling, J., Micke, P. (2011). Fresh Frozen Tissue: RNA Extraction and Quality Control. In: Dillner, J. (eds) Methods in Biobanking. Methods in Molecular Biology, vol 675. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-423-0_25
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DOI: https://doi.org/10.1007/978-1-59745-423-0_25
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Publisher Name: Humana Press, Totowa, NJ
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