Abstract
Two vital pre-requisites for genetic epidemiology have been fullfiled during the past decade and have led to a virtual explosion of knowledge concerning disease risks. Reliable databases over genetic variation derived from, e.g. the HUGO and HapMap projects, coupled with technological advances make large-scale genetic analyses and downstream bioinformatics suddenly affordable. Although recent prospective population-based biobanks have included DNA collection and purification in their planning, it is the older projects that currently are of greatest value due to the numbers of accumulated disease endpoints. In this chapter, methods to purify and use DNA derived from a variety of archival materials, including whole blood, formalin-fixed paraffin-embedded (FFPE) tissues, sera, dried blood spots (DBS), cervical cell suspensions, and mouthwash are presented and evaluated in a context of quality control guidelines to provide objective measure of the usefulness of various sample types for genetic epidemiology.
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Acknowledgements
The authors thank the Knut and Alice Wallenberg Foundation who through SWEGENE have financed the laboratory equipment, and Joakim Dillner, PI for the Cancer Control and Prevention using Registries and Biobanks (EU FP6) for economic support of MILS. We thank Anna Söderlund-Strand for access to her data on cervical cell suspensions, Maria Sterner and Liselotte Hall for help with the SEQUENOM MassArray analyses, and Sophia Harlid and Christina Gerouda for DNA extractions from serum on the MagNAPure.
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Ivarsson, M., Carlson, J. (2011). Extraction, Quantitation, and Evaluation of Function DNA from Various Sample Types. In: Dillner, J. (eds) Methods in Biobanking. Methods in Molecular Biology, vol 675. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-423-0_14
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DOI: https://doi.org/10.1007/978-1-59745-423-0_14
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