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Leukemia pp 179–206Cite as

Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 538))

Summary

Genetic abnormalities in leukaemia range from single gene defects to chromosomal translocations, inversions, losses and gains. While conventional technologies can detect macroscopic abnormalities, finding smaller regions remained a challenge until the recent introduction of high-resolution genomic platforms. Microarrays based on single nucleotide polymorphisms is one such technology. It has made possible genome-wide allelic association studies of predisposition to common clinical problems. This approach is also being used to identify somatic changes in cancer, such as loss, gain and copy-neutral loss of heterozygosity (CN-LOH), which are below the level of detection by conventional systems. Such arrays have been used to identify key genes involved in paediatric acute lymphoblastic leukaemia. We have used these arrays to identify regions of CN-LOH on a genome-wide scale in a large series of acute myeloid leukaemia samples, which so far has not been possible through any other technology.

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Acknowledgements

The work described in this chapter was supported by the grants from the Leukaemia Research Fund (05054 to Bryan D. Young) and Cancer Research UK (C6277/A6789 to Bryan D. Young).

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Authors and Affiliations

  1. Cancer Research UK Medical Oncology Unit, Barts and the London School of Medicine and Dentistry, Queen Mary College, University of London, London, UK

    Bryan D. Young

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  1. Manu Gupta
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  2. Bryan D. Young
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Correspondence to Bryan D. Young .

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Editors and Affiliations

  1. King's College London, The University of London, London, UK

    Chi Wai Eric So (Professor and Chair in Leukaemia Biology) (Professor and Chair in Leukaemia Biology)

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Gupta, M., Young, B. (2009). Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer. In: Eric So, C.W. (eds) Leukemia. Methods in Molecular Biology™, vol 538. Humana Press. https://doi.org/10.1007/978-1-59745-418-6_9

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  • DOI: https://doi.org/10.1007/978-1-59745-418-6_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-989-5

  • Online ISBN: 978-1-59745-418-6

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