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Detection of Replication Competent Retrovirus and Lentivirus

Protocol
Part of the Methods In Molecular Biology™ book series (MIMB, volume 506)

Summary

Retroviral vectors based on murine leukemia viruses (MuLV) have been used in clinical investigations for over a decade. Alternative retroviruses, most notably vectors based on HIV-1 and other lentiviruses, are now entering into clinical trials. Although vectors are designed to be replication defective, recombination events during vector production could lead to the generation of replication competent retroviruses (RCR) or replication competent lentiviruses (RCL). Careful screening of vector prior to human use must insure that patients are not inadvertently exposed to RCR or RCL. We describe methods capable of detecting low levels of virus contamination and discuss the current regulatory guidelines for screening gene therapy products intended for human use.

Key words

Retroviral vectors Lentiviral vectors Gene therapy Replication competent retrovirus Replication competent lentivirus 
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© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Medical and Molecular GeneticsIndiana University School of MedicineIndianapolisUSA

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