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Arthritis Research pp 105–119Cite as

Preparation of Mononuclear Cells from Synovial Tissue

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 135))

Abstract

In rheumatoid arthritis (RA), the synovium represents the predominant site of inflammation and joint destruction and is regarded as the key organ involved in disease pathogenesis. It has been studied in different ways over the last 30 yr, yielding information about the mechanisms involved in disease and remains the tool most proximal to understanding the pathogenesis of RA. This chapter outlines how both histological and in vitro studies of dissociated tissue played key roles in the development of biological anti-TNF-α therapy and provides detailed protocols used routinely in the laboratory to facilitate studies of RA synovium and its composite cell populations.

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Authors and Affiliations

  1. Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, London, UK

    Jonathan T. Beech & Fionula M. Brennan

Authors
  1. Jonathan T. Beech
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  2. Fionula M. Brennan
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Editor information

Editors and Affiliations

  1. The Kennedy Institute of Rheumatology, Imperial College London, London, UK

    Andrew P. Cope

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© 2007 Humana Press Inc., Totowa, NJ

About this protocol

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Beech, J.T., Brennan, F.M. (2007). Preparation of Mononuclear Cells from Synovial Tissue. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 135. Humana Press. https://doi.org/10.1007/978-1-59745-401-8_7

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  • DOI: https://doi.org/10.1007/978-1-59745-401-8_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-344-2

  • Online ISBN: 978-1-59745-401-8

  • eBook Packages: Springer Protocols

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