Animal Cell Biotechnology

Volume 24 of the series Methods in Biotechnology pp 457-473

Vaccine Production

State of the Art and Future Needs in Upstream Processing
  • Yvonne GenzelAffiliated withMax Planck Institute for Dynamics of Complex Technical Systems
  • , Udo ReichlAffiliated withMax Planck Institute for Dynamics of Complex Technical SystemsOtto-von-Guericke-Universität MagdeburgInstitute for Bioprocess Technology

* Final gross prices may vary according to local VAT.

Get Access


The production of viral vaccines in animal cell culture can be accomplished with primary, diploid, or continuous (transformed) cell lines. Each cell line, each virus type, and each vaccine definition requires a specific production and purification process. Media have to be selected as well as the production vessel, production conditions, and type of process. Here, we describe different issues that have to be considered during virus-production processes by discussing the influenza virus production in a microcarrier system in detail as an example. The use of serum-containing as well as serum-free media, but also the use of stirred tank bioreactors or wave bioreactors, is considered.

Key Words

Vaccine virus influenza large-scale production microcarrier Madin-Darby canine kidney cell adherent virus harvest virus quantification wave bioreactor stirred tank bioreactor