Abstract
Dendritic cells (DC) are key regulators of the immune system. They are capable of stimulating lymphocytes to generate potent cell-mediated and humoral immune responses against pathogens and tumor cells. DC not only activate lymphocytes, but can also educate T cells to tolerate self-antigens, thereby minimizing autoimmune reactions. Another peculiarity of the DC system is the large variety of subsets described, both in the human and in the mouse, according to surface phenotype and organ distribution. Different protocols have been developed to differentiate DC from total mouse bone marrow in vitro. Here, we describe the isolation of a specific DC progenitor population, referred to as preimmunocytes, and document protocols for their differentiation into various DC subsets.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lanzavecchia, A. and Sallusto, F. (2001) Regulation of T cell immunity by dendritic cells. Cell 106, 263–266.
Banchereau, J., Briere, F., Caux, C., et al. (2000) Immunobiology of dendritic cells. Annu. Rev. Immunol. 18, 767–811.
Banchereau, J. and Steinman, R. M. (1998) Dendritic cells and the control of immunity. Nature 392, 245–252.
Brocker, T., Riedinger, M., and Karjalainen, K. (1997) Targeted expression of major histocompatibility complex (MHC) class II molecules demonstrates that dendritic cells can induce negative but not positive selection of thymocytes in vivo. J. Exp. Med. 185, 541–550.
Shortman, K. and Heath, W. R. (2001) Immunity or tolerance? That is the question for dendritic cells. Nat. Immunol. 2, 988–989.
Shortman, K. and Liu, Y. J. (2002) Mouse and human dendritic cell subtypes. Nat. Rev. Immunol. 2, 151–161.
Steinman, R. M., Turley, S., Mellman, I., and Inaba, K. (2000) The induction of tolerance by dendritic cells that have captured apoptotic cells. J. Exp. Med. 191, 411–416.
Suss, G. and Shortman, K. (1996) A subclass of dendritic cells kills CD4 T cells via Fas/Fas-ligand-induced apoptosis. J. Exp. Med. 183, 1789–1796.
Cella, M., Sallusto, F., and Lanzavecchia, A. (1997) Origin, maturation and antigen presenting function of dendritic cells. Curr. Opin. Immunol. 9, 10–16.
Steinman, R. M. (1991) The dendritic cell system and its role in immunogenicity. Annu. Rev. Immunol. 9, 271–296.
Ardavin, C., (2003) Origin, precursors and differentiation of mouse dendritic cells. Nat. Rev. Immunol. 3, 582–590.
Ardavin, C., Wu, L., Li, L., and Shortman, K. (1993) Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population. Nature 362, 761–763.
Manz, M. G., Traver, D., Miyamoto, T., Weissman, I. L., and Akashi, K. (2001) Dendritic cell potentials of early lymphoid and myeloid progenitors. Blood 97, 3333–3341.
Traver, D., Akashi, K., Manz, M., et al. (2000) Development of CD8alpha-positive dendritic cells from a common myeloid progenitor. Science 290, 2152–2154.
Asselin-Paturel, C., Boonstra, A., Dalod, M., et al. (2001) Mouse type I IFN-pro ducing cells are immature APCs with plasmacytoid morphology. Nat. Immunol. 2, 1144–1150.
Grouard, G., Rissoan, M. C., Filgueira, L., Durand, I., Banchereau, J., and Liu, Y. J. (1997) The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligand. J. Exp. Med. 185, 1101–1111.
Inaba, K., Inaba, M., Romani, N., et al. (1992) Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176, 1693–1702.
Lutz, M. B., Kukutsch, N., Ogilvie, A. L., et al. (1999) An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Methods 223, 77–92.
Scheicher, C., Mehlig, M., Zecher, R., and Reske, K. (1992) Dendritic cells from mouse bone marrow: in vitro differentiation using low doses of recombinant granulocyte-macrophage colony-stimulating factor. J. Immunol. Methods 154, 253–264.
Yamaguchi, Y., Tsumura, H., Miwa, M., and Inaba, K., (1997) Contrasting effects of TGF-beta 1 and TNF-alpha on the development of dendritic cells from progenitors in mouse bone marrow. Stem Cells 15, 144–153.
Brawand, P., Fitzpatrick, D. R., Greenfield, B. W., Brasel, K., Maliszewski, R., and De Smedt, T. (2002) Murine plasmacytoid predendritic cells generated from Flt3 ligand-supplemented bone marrow cultures are immature APCs. J. Immunol. 169, 6711–6719.
Gilliet, M., Boonstra, A., Paturel, C., et al. (2002) The development of murine plasmacytoid dendritic cell precursors is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 195, 953–958.
Inaba, K., Inaba, M., Romani, N., et al. (1992) Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176, 1693–1702.
Bruno, L., Seidl, T., and Lanzavecchia, A. (2001) Mouse pre-immunocytes as non-proliferating multipotent precursors of macrophages, interferon-producing cells, CD8alpha(+) and CD8alpha(−) dendritic cells. Eur. J. Immunol. 31, 3403–3412.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Bruno, L. (2007). Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow. In: Fairchild, P.J. (eds) Immunological Tolerance. Methods in Molecular Biology™, vol 380. Humana Press. https://doi.org/10.1007/978-1-59745-395-0_3
Download citation
DOI: https://doi.org/10.1007/978-1-59745-395-0_3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-652-8
Online ISBN: 978-1-59745-395-0
eBook Packages: Springer Protocols