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Mitochondria pp 107-123 | Cite as

Neurospora crassa as a Model Organism for Mitochondrial Biogenesis

  • Frank E. Nargang
  • Doron Rapaport
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

Neurospora crassa has proven to be an excellent organism for studying various aspects of the biology of mitochondria by biochemical and genetic approaches. As N. crassa is an obligate aerobe and contains complex I, its mitochondria are more similar to mammalian mitochondria than those of yeast. The recent sequencing of the genome of N. crassa and a gene knockout project that is under way make the organism even more attractive. We describe some of the advantages of N. crassa as a model organism and present methods for isolation of mitochondria, fractionation of these organelles, and disruption of essential genes in this organism.

Key Words

Digitonin fractionation Neurospora crassa sheltered disruption 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Frank E. Nargang
    • 1
  • Doron Rapaport
    • 2
  1. 1.Department of Biological SciencesUniversity of AlbertaEdmontonCanada
  2. 2.Institut für Physiologische Chemie der Universität MünchenMünchenGermany

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