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Isolation of Mitochondria From Procyclic Trypanosoma brucei

  • André Schneider
  • Fabien Charrière
  • Mascha Pusnik
  • Elke K. Horn
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

The mitochondrion of the parasitic protozoon Trypanosoma brucei shows a number of unique features, many of which represent highly interesting research topics. Studies of these subjects require the purification of mitochondrial fractions. Here, we describe and discuss the two most commonly used methods to isolate mitochondria from insect stage T. brucei. In the first protocol, the cells are lysed under hypotonic conditions, and mitoplast vesicles are isolated on Percoll gradients; in the second method, lysis occurs isotonically by N2 cavitation, and the mitochondrial vesicles are isolated by Nycodenz gradient centrifugation.

Key Words

N2 cavitation Nycodenz Percoll subcellular fractionation trypanosome Trypanosoma brucei 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • André Schneider
    • 1
  • Fabien Charrière
    • 1
  • Mascha Pusnik
    • 1
  • Elke K. Horn
    • 1
  1. 1.Department of BiologyUniversity of FribourgFribourgSwitzerland

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