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Mitochondria pp 559-571 | Cite as

Arabidopsis Mitochondrial Proteomics

  • Joshua L. Heazlewood
  • Harvey Millar A. 
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

Significant efforts have sought to uncover the protein profile of Arabidopsis mitochondria to act as a model for the mitochondrial proteome from plants. A combination of techniques have been undertaken to achieve this goal. We outline a basic two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation of mitochondrial proteins, in-gel trypsination techniques, complex protein lysate digestions, and the identification of proteins by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry.

Key Words

Arabidopsis mass spectrometry proteomics 2D-PAGE 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Joshua L. Heazlewood
    • 1
  • Harvey Millar A. 
    • 2
  1. 1.ARC Centre of Excellence in Plant Energy Biology and School of Biomedical and Chemical SciencesThe University of Western AustraliaCrawleyAustralia
  2. 2.ARC Centre of Excellence in Plant Energy Biology and School of Biomedical and Chemical SciencesThe University of Western AustraliaPerthAustralia

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