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Mitochondria pp 485-501 | Cite as

Mitochondrial Division in Caenorhabditis elegans

  • Shilpa Gandre
  • Alexander M. van der Bliek
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

The study of mitochondrial division proteins has largely focused on yeast and mammalian cells. We describe methods to use Caenorhabditis elegans as an alternative model for studying mitochondrial division, taking advantage of the many wonderful resources provided by the C. elegans community. Our methods are largely based on manipulation of gene expression using classic and molecular genetic techniques combined with fluorescence microscopy. Some biochemical methods are also included. As antibodies become available, these biochemical methods are likely to become more sophisticated.

Key Words

C. elegans division Drp1 fusion nematode 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Shilpa Gandre
    • 1
  • Alexander M. van der Bliek
    • 1
  1. 1.Department of Biological ChemistryDavid Geffen School of Medicine at UCLALos Angeles

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