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Mitochondria pp 433-459 | Cite as

Fluorescence Imaging of Mitochondria in Yeast

  • Theresa C. Swayne
  • Anna C. Gay
  • Liza A. Pon
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model eukaryote. However, it is now feasible for laboratories to acquire and analyze high-resolution, multidimensional images of yeast cell biology, including the mitochondria. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing yeast mitochondria using fluorescence microscopy.

Key Words

Deconvolution fluorescent proteins immunofluorescence live-cell imaging microscopy vital staining yeast 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Theresa C. Swayne
    • 1
  • Anna C. Gay
    • 1
  • Liza A. Pon
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of Physicians and SurgeonsColumbia UniversityNew York

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