Fluorescence Imaging of Mitochondria in Yeast
The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model eukaryote. However, it is now feasible for laboratories to acquire and analyze high-resolution, multidimensional images of yeast cell biology, including the mitochondria. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing yeast mitochondria using fluorescence microscopy.
Key WordsDeconvolution fluorescent proteins immunofluorescence live-cell imaging microscopy vital staining yeast
- 11.Nunnari, J., Marshall, W. F., Straight, A., Murray, A., Sedat, J. W., and Walter, P. (1997) Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA. Mol. Biol. Cell. 8, 1233–1242.PubMedGoogle Scholar
- 13.Wallace, W., Schaefer, L. H., and Swedlow, J. R. (2001) A workingperson’s guide to deconvolution in light microscopy. Biotechnology 31, 1076–1078.Google Scholar
- 20.Stevens, B. (1977) Variation in number and volume of the mitochondria in yeast according to growth conditions. A study based on serial sectioning and computer graphics reconstitution. Biol. Cell. 28, 37–56.Google Scholar