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Mitochondria pp 389-403 | Cite as

Oxidative Stress and Plant Mitochondria

  • Nicolas L. Taylor
  • A. Harvey Millar
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

Mitochondria not only are a source of reactive oxygen species (ROS) but also are sites of oxidative damage. In plants, mitochondria must normally operate when there are high levels of ROS produced during photosynthesis and photorespiration. These levels are further enhanced during biotic and abiotic stress of plants. Excessive stress can lead to mitochondrial damage, which may then lead to induction of programmed cell death in plants. We outline methods for imposing oxidative stress in plants, provide methods for measurements of its severity, and then explain assays for assessing plant mitochondrial oxidative damage and measuring the capacity of key stress defense and response pathways.

Key Words

Aconitase alternative oxidase glycine decarboxylase lipid peroxidation lipoic acid reactive oxygen species superoxide dismutase 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Nicolas L. Taylor
    • 1
  • A. Harvey Millar
    • 2
  1. 1.Department of Plant SciencesUniversity of OxfordOxfordUK
  2. 2.ARC Centre of Excellence in Plant Energy Biology and School of Biomedical and Chemical SciencesThe University of Western AustraliaPerthAustralia

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