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The Mitochondria of Cultured Mammalian Cells

I: Analysis by Immunofluorescence Microscopy, Histochemistry, Subcellular Fractionation, and Cell Fusion

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 372))

Abstract

Mitochondria form a dynamic network in which continuous movement, fusion, and division ensure the distribution and exchange of proteins and deoxyribonucleic acid (DNA). The recent past has seen the identification and characterization of the first proteins governing the organization, function, and dynamics of mitochondria and mitochondrial DNA, and it is predictable that numerous new proteins will require localization and functional characterization in the future. In this chapter, we describe methods for the visualization of mitochondria and mitochondrial activity in cultured mammalian cells to establish the localization or distribution of its components and to study mitochondrial fusion.

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© 2007 Humana Press Inc., Totowa, NJ

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Malka, F., Auré, K., Goffart, S., Spelbrink, J.N., Rojo, M. (2007). The Mitochondria of Cultured Mammalian Cells. In: Leister, D., Herrmann, J.M. (eds) Mitochondria. Methods in Molecular Biology™, vol 372. Humana Press. https://doi.org/10.1007/978-1-59745-365-3_1

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  • DOI: https://doi.org/10.1007/978-1-59745-365-3_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-667-2

  • Online ISBN: 978-1-59745-365-3

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