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The Mitochondria of Cultured Mammalian Cells

I: Analysis by Immunofluorescence Microscopy, Histochemistry, Subcellular Fractionation, and Cell Fusion
  • Florence Malka
  • Karine Auré
  • Steffi Goffart
  • Johannes N. Spelbrink
  • Manuel Rojo
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)

Abstract

Mitochondria form a dynamic network in which continuous movement, fusion, and division ensure the distribution and exchange of proteins and deoxyribonucleic acid (DNA). The recent past has seen the identification and characterization of the first proteins governing the organization, function, and dynamics of mitochondria and mitochondrial DNA, and it is predictable that numerous new proteins will require localization and functional characterization in the future. In this chapter, we describe methods for the visualization of mitochondria and mitochondrial activity in cultured mammalian cells to establish the localization or distribution of its components and to study mitochondrial fusion.

Key Words

Cytochrome-c oxidase mitochondrial fusion mitochondrial morphology succinate dehydrogenase 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Florence Malka
    • 1
  • Karine Auré
    • 1
  • Steffi Goffart
    • 2
  • Johannes N. Spelbrink
    • 2
  • Manuel Rojo
    • 1
  1. 1.INSERM U582 - Institut de MyologieUniversité Pierre et Marie Curie, IFR14, Groupe Hospitalier Pitié-SalpêtrièreParis CedexFrance
  2. 2.FinMIT Centre of ExcellenceInstitute of Medical Technology and Tampere University Hospital, University of TampereTampereFinland

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