Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2

  • Nicholas R. Staten
  • Beverly A. Reitz
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 644)

Abstract

Cyclooxygenase (COX) enzymes play important roles in normal physiology and during inflammation of various cells and tissues. In order to help understand the functions of these enzymes, their genes can be cloned to facilitate the production of the proteins in recombinant form. We outline a method to clone the genes from a human macrophage cell line for expression in an insect cell line infected with recombinant baculovirus encoding these enzymes.

Key words

Cyclooxygenase (COX) Messenger ribonucleic acid (mRNA) Polymerase chain reaction (PCR) Baculovirus Bacmid Insect cells 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Nicholas R. Staten
    • 1
  • Beverly A. Reitz
    • 1
  1. 1.Pfizer, Inc.ChesterfieldUSA

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