Abstract
Cyclooxygenase-2 (COX-2), the rate-limiting enzyme for prostaglandin (PG) biosynthesis, plays a key role in inflammation, tumorigenesis, development and circulatory homeostasis. COX-2 expression is rapidly and sensitively regulated by various stimuli, and also its regulation is distinct among cell types at transcriptional and posttranscriptional levels. Therefore, it is important to consider these features of COX-2 expression in the reporter assays we describe in this chapter. Emphasis should be made with regard to two points. Firstly, COX-2 reporter assays should be evaluated by intrinsic COX-2 expression, such as RT-PCR, northern blotting, western blotting, or by PGE2 measurement. Secondly, one must carefully choose several conditions in the reporter assays for experimental purposes.
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Acknowledgments
We would like to thank Ms. Haruka Takeuchi, Mariko Hotta, Tomoko Tsukamoto, and Tomomi Matsuyama for their technical assistance. This work was supported by Grant-in-Aid for scientific research from Ministry of Education, Culture, Sports, Science and Technology (Houga18650213, B19300250).
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Inoue, H., Nakata, R. (2010). Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites. In: Ayoub, S., Flower, R., Seed, M. (eds) Cyclooxygenases. Methods in Molecular Biology, vol 644. Humana Press. https://doi.org/10.1007/978-1-59745-364-6_2
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DOI: https://doi.org/10.1007/978-1-59745-364-6_2
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